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human pd 1 fc chimera protein  (R&D Systems)


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    Structured Review

    R&D Systems human pd 1 fc chimera protein
    Human Pd 1 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pd+1+fc+protein/Recombinant+Human+PD-1+Fc+Chimera+Protein%2C+CF/pm41872151-167-46-52
    Average 96 stars, based on 95 article reviews
    human pd 1 fc chimera protein - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: CircRHBDD1 promotes immune escape via IGF2BP2/PD-L1 signaling and acts as a nanotherapeutic target in gastric cancer.
    Article Snippet: .. After 24 h, cells were fixed with 4% paraformaldehyde for 15 min, followed by treatment with recombinant human PD-1 Fc protein (R&D Systems, USA) for 1 h at room temperature. .. Subsequently, cells were incubated with anti-human Alexa Fluor 488 dye (Invitrogen, USA) for 1 h. Nuclei were stained with DAPI for 5 min. Fluorescence intensity of Alexa Fluor 488 was measured using a Synergy Neo microplate reader (BioTeK, VT, USA) and normalized to total protein content.

    Article Title: The covalent modification of STAT1 cysteines by sulforaphane promotes antitumor immunity via blocking IFN-γ-induced PD-L1 expression.
    Article Snippet: .. Subsequently, the cells were incubated with recombinant human PD-1 Fc protein (R&D Systems). ..

    Article Title: δ-Tocotrienol Potentiates Breast and Prostate Cancer Cells to Paclitaxel via Suppressing PD-L1-Mediated Cancer-Promoting Signaling.
    Article Snippet: Vitamin E can exert either a cancer preventive effect or improve the therapeutic efficacy of chemotherapeutic drugs against multiple types of cancer.. Ample evidence suggests that the cancer preventive activity of vitamin E is formdependent; however, it is not clear whether its chemosensitization effect is also influenced by its forms.. The objectives of this study were to investigate whether the eight natural forms of vitamin E produced differential sensitization effects on cancer chemotherapeutic drugs and to address whether the chemosensitization effect of vitamin E was associated with its inhibitory effect on programmed cell death ligand 1 (PDL1) signaling.

    Article Title: CircRHBDD1 promotes immune escape via IGF2BP2/PD-L1 signaling and acts as a nanotherapeutic target in gastric cancer
    Article Snippet: .. After 24 h, cells were fixed with 4% paraformaldehyde for 15 min, followed by treatment with recombinant human PD-1 Fc protein (R&D Systems, USA) for 1 h at room temperature. .. Subsequently, cells were incubated with anti-human Alexa Fluor 488 dye (Invitrogen, USA) for 1 h. Nuclei were stained with DAPI for 5 min. Fluorescence intensity of Alexa Fluor 488 was measured using a Synergy Neo microplate reader (BioTeK, VT, USA) and normalized to total protein content.

    Article Title: The covalent modification of STAT1 cysteines by sulforaphane promotes antitumor immunity via blocking IFN-γ-induced PD-L1 expression
    Article Snippet: .. Subsequently, the cells were incubated with recombinant human PD-1 Fc protein (R&D Systems). ..

    Article Title: Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC
    Article Snippet: .. For PD-L1/PD-1 binding assessment, samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight, followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). .. For comparing tumor cell growth in different groups, tumor sections in a cryostat were incubated with either anti-Ki67 (1:200, ab15580, Abcam) or anti-cleaved CASP3 (1:200, ab32042, Abcam), along with anti-PanCK (1:200, ab7753, Abcam) primary antibody at 4 °C overnight, followed by a 2 h exposure to Alexa Fluor 555-conjugated anti-rabbit secondary antibody (1:200, A0453, Beyotime) and Cy5-conjugated anti-mouse secondary antibody (1:200, 15-175-166, Jackson ImmunoResearch), shielded from light.

    Article Title: Lactylation-related gene signature accurately predicts prognosis and immunotherapy response in gastric cancer
    Article Snippet: .. GC cells were incubated with recombinant human PD-1 Fc protein (R&D Systems) for 1 hour. .. Subsequently, anti-human Alexa Fluor 488 dye-conjugated secondary antibodies (Life Technologies) were applied for 1 hour.

    Incubation:

    Article Title: The covalent modification of STAT1 cysteines by sulforaphane promotes antitumor immunity via blocking IFN-γ-induced PD-L1 expression.
    Article Snippet: .. Subsequently, the cells were incubated with recombinant human PD-1 Fc protein (R&D Systems). ..

    Article Title: δ-Tocotrienol Potentiates Breast and Prostate Cancer Cells to Paclitaxel via Suppressing PD-L1-Mediated Cancer-Promoting Signaling.
    Article Snippet: Vitamin E can exert either a cancer preventive effect or improve the therapeutic efficacy of chemotherapeutic drugs against multiple types of cancer.. Ample evidence suggests that the cancer preventive activity of vitamin E is formdependent; however, it is not clear whether its chemosensitization effect is also influenced by its forms.. The objectives of this study were to investigate whether the eight natural forms of vitamin E produced differential sensitization effects on cancer chemotherapeutic drugs and to address whether the chemosensitization effect of vitamin E was associated with its inhibitory effect on programmed cell death ligand 1 (PDL1) signaling.

    Article Title: The covalent modification of STAT1 cysteines by sulforaphane promotes antitumor immunity via blocking IFN-γ-induced PD-L1 expression
    Article Snippet: .. Subsequently, the cells were incubated with recombinant human PD-1 Fc protein (R&D Systems). ..

    Article Title: PKC α inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability
    Article Snippet: .. To measure the interaction between PD-1 and PD-L1, cells were fixed in 4% paraformaldehyde at room temperature for 20 min and then incubated with human PD-1 Fc protein (R&D Systems) for 2 h, followed by incubation with anti-human Alexa Fluor 488 secondary antibodies (Life Technologies) at room temperature for 1 h. After being incubated with DAPI, the cells were visualized with a confocal fluorescence microscope (Olympus, CA, USA). .. C57BL/6-Tg(TcraTcrb)1100Mjb/J mice (OT-I mice) were purchased from Shanghai Biomodel Organism Science & Technology Development Co., Ltd.

    Article Title: Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC
    Article Snippet: .. For PD-L1/PD-1 binding assessment, samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight, followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). .. For comparing tumor cell growth in different groups, tumor sections in a cryostat were incubated with either anti-Ki67 (1:200, ab15580, Abcam) or anti-cleaved CASP3 (1:200, ab32042, Abcam), along with anti-PanCK (1:200, ab7753, Abcam) primary antibody at 4 °C overnight, followed by a 2 h exposure to Alexa Fluor 555-conjugated anti-rabbit secondary antibody (1:200, A0453, Beyotime) and Cy5-conjugated anti-mouse secondary antibody (1:200, 15-175-166, Jackson ImmunoResearch), shielded from light.

    Article Title: Lactylation-related gene signature accurately predicts prognosis and immunotherapy response in gastric cancer
    Article Snippet: .. GC cells were incubated with recombinant human PD-1 Fc protein (R&D Systems) for 1 hour. .. Subsequently, anti-human Alexa Fluor 488 dye-conjugated secondary antibodies (Life Technologies) were applied for 1 hour.

    Multiple Displacement Amplification:

    Article Title: δ-Tocotrienol Potentiates Breast and Prostate Cancer Cells to Paclitaxel via Suppressing PD-L1-Mediated Cancer-Promoting Signaling.
    Article Snippet: Vitamin E can exert either a cancer preventive effect or improve the therapeutic efficacy of chemotherapeutic drugs against multiple types of cancer.. Ample evidence suggests that the cancer preventive activity of vitamin E is formdependent; however, it is not clear whether its chemosensitization effect is also influenced by its forms.. The objectives of this study were to investigate whether the eight natural forms of vitamin E produced differential sensitization effects on cancer chemotherapeutic drugs and to address whether the chemosensitization effect of vitamin E was associated with its inhibitory effect on programmed cell death ligand 1 (PDL1) signaling.

    Fluorescence:

    Article Title: PKC α inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability
    Article Snippet: .. To measure the interaction between PD-1 and PD-L1, cells were fixed in 4% paraformaldehyde at room temperature for 20 min and then incubated with human PD-1 Fc protein (R&D Systems) for 2 h, followed by incubation with anti-human Alexa Fluor 488 secondary antibodies (Life Technologies) at room temperature for 1 h. After being incubated with DAPI, the cells were visualized with a confocal fluorescence microscope (Olympus, CA, USA). .. C57BL/6-Tg(TcraTcrb)1100Mjb/J mice (OT-I mice) were purchased from Shanghai Biomodel Organism Science & Technology Development Co., Ltd.

    Microscopy:

    Article Title: PKC α inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability
    Article Snippet: .. To measure the interaction between PD-1 and PD-L1, cells were fixed in 4% paraformaldehyde at room temperature for 20 min and then incubated with human PD-1 Fc protein (R&D Systems) for 2 h, followed by incubation with anti-human Alexa Fluor 488 secondary antibodies (Life Technologies) at room temperature for 1 h. After being incubated with DAPI, the cells were visualized with a confocal fluorescence microscope (Olympus, CA, USA). .. C57BL/6-Tg(TcraTcrb)1100Mjb/J mice (OT-I mice) were purchased from Shanghai Biomodel Organism Science & Technology Development Co., Ltd.

    Binding Assay:

    Article Title: Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC
    Article Snippet: .. For PD-L1/PD-1 binding assessment, samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight, followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). .. For comparing tumor cell growth in different groups, tumor sections in a cryostat were incubated with either anti-Ki67 (1:200, ab15580, Abcam) or anti-cleaved CASP3 (1:200, ab32042, Abcam), along with anti-PanCK (1:200, ab7753, Abcam) primary antibody at 4 °C overnight, followed by a 2 h exposure to Alexa Fluor 555-conjugated anti-rabbit secondary antibody (1:200, A0453, Beyotime) and Cy5-conjugated anti-mouse secondary antibody (1:200, 15-175-166, Jackson ImmunoResearch), shielded from light.



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    R&D Systems human pd1 fc protein
    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    BIEXO@IPA platform for enhanced CAR-T therapy in lung cancer. In this study, iPSCs were lentivirally transduced with anti-PD-1/MSLN scFv to generate iPSC lines expressing the engineered construct (BiPSC). BIEXOs were isolated and concentrated from BiPSC culture supernatant via ultracentrifugation, then loaded with IPA through electroporation to form BIEXO@IPA. This platform was nebulized into orthotopic lung cancer mouse models for targeted delivery. In the lung TME, BIEXO@IPA bridges endogenous PD-1 + CD8 + T/CAR-T cells to MSLN-expressing tumor cells while blocking PD-1/PD-L1 signaling and exerting antitumor effects via : (1) BIEXO reverses tumor cell malignancy; (2) IPA delivery enhances Tpex and effector T cells (Teff); (3) activated Teff-mediated cytotoxicity

    Journal: Journal of Nanobiotechnology

    Article Title: Engineering BiTE-inspired IPSC-exosomes to potentiate CAR-T cell therapy against lung cancer

    doi: 10.1186/s12951-026-04242-3

    Figure Lengend Snippet: BIEXO@IPA platform for enhanced CAR-T therapy in lung cancer. In this study, iPSCs were lentivirally transduced with anti-PD-1/MSLN scFv to generate iPSC lines expressing the engineered construct (BiPSC). BIEXOs were isolated and concentrated from BiPSC culture supernatant via ultracentrifugation, then loaded with IPA through electroporation to form BIEXO@IPA. This platform was nebulized into orthotopic lung cancer mouse models for targeted delivery. In the lung TME, BIEXO@IPA bridges endogenous PD-1 + CD8 + T/CAR-T cells to MSLN-expressing tumor cells while blocking PD-1/PD-L1 signaling and exerting antitumor effects via : (1) BIEXO reverses tumor cell malignancy; (2) IPA delivery enhances Tpex and effector T cells (Teff); (3) activated Teff-mediated cytotoxicity

    Article Snippet: Recombinant human PD-1 protein (5 μg/mL, R&D Systems, 1086-PD) was used for capture.

    Techniques: Transduction, Expressing, Construct, Isolation, Electroporation, Blocking Assay

    Functional characterization and T-cell bridging activity. ( a , b ) Binding affinity analysis of engineered exosomes (MIEXO, PIEXO, BIEXO) to MSLN (a) and PD-1 ( b ) via indirect ELISA. Exosomes were added to PD-1 or MSLN-coated plates and detected via an HRP-conjugated anti-His-tag antibody. ( c , d ) Flow cytometric analysis of engineered exosomes (100 µg/mL) binding to PD-1 + T cells ( c ) and LLC-MSLN cells ( d ), with His-tag mean fluorescence intensity (MFI) quantification (right panel). ( e , f ) Confocal microscopy analysis of BIEXO targeting to PD-1 + T cells ( e ) and LLC-MSLN cells ( f ). ( g ) Representative confocal microscopy images and corresponding quantification showing BIEXO-mediated bridging between PD-1 + T cells and LLC-MSLN cells. ( h ) FRET analysis of dose-dependent bridging efficiency. ( i , j ) Prevention of PD-L1-induced T-cell exhaustion measured by LDH release ( n = 3). ( k , l ) Experimental design (k) for ex vivo culture of CD8 + T cells isolated from mouse spleen, and flow cytometric analysis of TCF-1 + Tpex cell expansion in PD-1 + CD8 + T cells following pre-treatment with the AhR antagonist CH-223,191 and BIEXO@IPA stimulation ( l ). ( m ) In vitro growth inhibition of LLC-MSLN cells following treatment. ( n ) Enhanced cytotoxicity of TILs isolated from LLC-MSLN tumor-bearing mice against LLC-MSLN cells following BIEXO@IPA treatment. Data are presented as mean ± SD. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (c, d, j, m, n). * p < 0.05 , ** p < 0.01 , *** p < 0.001 , **** p < 0.0001

    Journal: Journal of Nanobiotechnology

    Article Title: Engineering BiTE-inspired IPSC-exosomes to potentiate CAR-T cell therapy against lung cancer

    doi: 10.1186/s12951-026-04242-3

    Figure Lengend Snippet: Functional characterization and T-cell bridging activity. ( a , b ) Binding affinity analysis of engineered exosomes (MIEXO, PIEXO, BIEXO) to MSLN (a) and PD-1 ( b ) via indirect ELISA. Exosomes were added to PD-1 or MSLN-coated plates and detected via an HRP-conjugated anti-His-tag antibody. ( c , d ) Flow cytometric analysis of engineered exosomes (100 µg/mL) binding to PD-1 + T cells ( c ) and LLC-MSLN cells ( d ), with His-tag mean fluorescence intensity (MFI) quantification (right panel). ( e , f ) Confocal microscopy analysis of BIEXO targeting to PD-1 + T cells ( e ) and LLC-MSLN cells ( f ). ( g ) Representative confocal microscopy images and corresponding quantification showing BIEXO-mediated bridging between PD-1 + T cells and LLC-MSLN cells. ( h ) FRET analysis of dose-dependent bridging efficiency. ( i , j ) Prevention of PD-L1-induced T-cell exhaustion measured by LDH release ( n = 3). ( k , l ) Experimental design (k) for ex vivo culture of CD8 + T cells isolated from mouse spleen, and flow cytometric analysis of TCF-1 + Tpex cell expansion in PD-1 + CD8 + T cells following pre-treatment with the AhR antagonist CH-223,191 and BIEXO@IPA stimulation ( l ). ( m ) In vitro growth inhibition of LLC-MSLN cells following treatment. ( n ) Enhanced cytotoxicity of TILs isolated from LLC-MSLN tumor-bearing mice against LLC-MSLN cells following BIEXO@IPA treatment. Data are presented as mean ± SD. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (c, d, j, m, n). * p < 0.05 , ** p < 0.01 , *** p < 0.001 , **** p < 0.0001

    Article Snippet: Recombinant human PD-1 protein (5 μg/mL, R&D Systems, 1086-PD) was used for capture.

    Techniques: Functional Assay, Activity Assay, Binding Assay, Indirect ELISA, Fluorescence, Confocal Microscopy, Ex Vivo, Isolation, In Vitro, Inhibition

    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer

    doi: 10.1111/jcmm.70947

    Figure Lengend Snippet: DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Article Snippet: Cells in each group were fixed in 4% PFA for 15 min, incubated with recombinant human PD1 Fc protein (R&D Systems, Minneapolis, MN, USA) for 1 h, and then incubated with anti‐human Alexa Fluor 488 secondary antibodies (Thermal Fisher Scientific) for 1 h at room temperature.

    Techniques: Fluorescence, Staining, Cell Culture, Negative Control